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Hisatake Koji

Affiliation
Faculty of Medicine
Official title
Professor
URL
Email
 
Fax
029-853-3929
Research fields
General medical chemistry
Biomolecular chemistry
Research keywords
Transcription
Gene Expression
Chromatin
Mechanisms of Transcriptional Regulation
iPS Cell
Regenerative Medicine
Research projects
Tcl1を利用したゲノム変異の少ないiPS細胞誘導方法の開発2017 -- 2018久武 幸司Japan Society for the Promotion of Science/Challenging Research (Exploratory)6370000
iPS細胞誘導過程でのKlf4による細胞形態と細胞機能のリプログラミング機構2017 -- 2020久武 幸司Japan Society for the Promotion of Science/Grant-in-Aid for Scientific Research(B)17680000
iPS細胞誘導の初期段階での転写因子ネットワークの解析2013-04 -- 2016-03Koji HisatakeJapan Society of for the Promotion of Science/Grant-in-Aid for Scientific Research5200000
新規コアクチベーターNF複合体の構成因子と制御機構の解析2010-04 -- 2013-03久武幸司Japan Society of for the Promotion of Science/基盤研究(C)4580000
リン酸化酵素によるクロマチン修飾の制御機構の解明                2006 -- 2007久武幸司Japan Society of for the Promotion of Science/基盤研究(C)3890000
新規コアクチベーターによる転写過程の包括的調節機構の解析            2006 -- 2007久武幸司Ministry of Education, Culture, Sports, Science and Technology, Japan/特定領域研究8700000
Career history
1991-04 -- 1994-03ロックフェラー大学 ポストドクトラル フェロー
1994-04 -- 1994-10ロックフェラー大学 ポストドクトラル アソシエート
1994-11 -- 2000-05埼玉医科大学 医学部 講師
2000-06 -- 2005-12埼玉医科大学 医学部 助教授
2006-01 -- 2011-09筑波大学 人間総合科学研究科 教授
2011-10 -- (current)筑波大学 医学医療系 教授
Academic background
-- 1985岡山大学 医学部 医学科Graduated
-- 1991東京大学大学院 医学系研究科 第二基礎医学Completed
Degree
医学博士東京大学
Academic societies
2013-01 -- (current)THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE
2002 -- (current)THE JAPANESE BIOCHEMICAL SOCIETY
1995 -- (current)The Molecular Biology Society of Japan
2003 -- (current)American Society for Biochemistry and Molecular Biology
Articles
  • Manipulation of KLF4 expression generates iPSCs paused at successive stages of reprogramming
    Ken Nishimura; Tetsuo Kato; Chen Chen; Lalhaba Oinam; Emi...
    Stem Cell Reports, 2014-11
  • Klf4遺伝子発現調節によるiPS細胞誘導中間体の作製
    西村 健; 久武幸司
    医学のあゆみ/255(2)/pp.173-174, 2015-10
  • The RNA Binding Complexes NF45-NF90 and NF45-NF110 Associate Dynamically with the c-fos Gene and Function as Transcriptional Coactivators
    Nakadai Tomoyoshi; Fukuda Aya; Shimada Miho; Nishimura Ke...
    JOURNAL OF BIOLOGICAL CHEMISTRY/290(44)/pp.26832-26845, 2015-10
  • Association of the winged helix motif of the TFIIE alpha subunit of TFIIE with either the TFIIE beta subunit or TFIIB distinguishes its functions in transcription
    Tanaka Aki; Akimoto Yusuke; Kobayashi Satoko; Hisatake Ko...
    GENES TO CELLS/20(3)/pp.203-216, 2015-03
  • The Paired-box Homeodomain Transcription Factor Pax6 Binds to the Upstream Region of the TRAP Gene Promoter and Suppresses Receptor Activator of NF-kappa B Ligand (RANKL)-induced Osteoclast Differentiation
    Kogawa Masakazu; Hisatake Koji; Atkins Gerald J.; Findlay...
    JOURNAL OF BIOLOGICAL CHEMISTRY/288(43)/pp.31299-31312, 2013-10
  • Manipulation of KLF4 expression generates iPSCs paused at successive stages of reprogramming
    Ken Nishimura; Tetsuo Kato; Chen Chen; Lalhaba Oinam; Emi...
    Stem Cell Reports/3(5)/pp.915-929, 2014-11
  • The Paired-box Domain Transcription Factor Pax6 Binds to the Upstream Region of the TRAP Gene Promoter and Suppresses RANKL-induced Osteoclast Differentiation.
    FUKUDA AYA; Kogawa M; Hisatake K; Atkins GJ; Findlay DM; ...
    Journal of Biological Chemistry/288(43)/pp.31299-31312, 2013-10
  • Heterogeneous Nuclear Ribonucleoprotein R Cooperates with Mediator to Facilitate Transcription Reinitiation on the c-Fos Gene
    Fukuda Aya; Shimada Miho; Nakadai Tomoyoshi; Nishimura Ke...
    PLOS ONE/8(8), 2013-08
  • DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation
    Chen Yexi; Yamaguchi Yuki; Tsunego Yuta; Yamamoto Junichi...
    Genes & Development/23(23)/pp.2765-2777, 2009-12
  • Immunoelectron microscopy of adult T cell leukemia-associated antigen by the peroxidase-labeled antibody method.
    Oda T; Watanabe S; Araki Y; Hatsushika M; Sumi H; Nakamur...
    Cellular and Molecular Biology/31(1)/p.49-56, 1985-01
  • Sequence-specific binding of a transcription factor TFID to the promoter region of mouse ribosomal RNA gene.
    Tanaka N; Kato H; Ishikawa Y; Hisatake K; Tashiro K; Komi...
    The Journal of Biological Chemistry/256(23)/p.12836-13842, 1990-08
  • Structure of the core promoter of human and mouse ribosomal RNA gene: Asymmetry of species-specific transcription.
    Ishikawa Y; Safrany G; Hisatake K; Tanaka N; Maeda Y; Kat...
    Journal of Molecular Biology/218(1)/p.55-67, 1991-03
  • Cloning and structural analysis of cDNA and its gene for mouse transcription factor UBF.
    Hisatake K; Nishimura T; Maeda Y; Hanada K; Song C; Muram...
    Nucleic Acids Research/19(17)/p.4631 4637, 1991-09
  • Sequence of general transcription factor TFIIB and relationships to other initiation factors.
    Malik S; Hisatake K; Sumimoto H; Horikoshi M; Roeder RG
    Proceedings of the National Academy of Sciences of the United States of America/88(21)/p.9553-9557, 1991-11
  • Conserved structural motifs between Xenopus and human TFIIB.
    Hisatake K; Malik S; Roeder RG; Horikoshi M
    Nucleic Acids Research/19(23)/p.6639, 1991-12
  • Isolation and characterization of a cDNA encoding Drosophila transcription factor TFIIB.
    Yamashita S; Wada K; Horikoshi M; Gong D-W; Kokubo T; His...
    Proceedings of the National Academy of Sciences of the United States of America/89(7)/p.2839-2843, 1992-04
  • Mouse rRNA transcription factor mUBF requires both HMG-box1 and an acidic tail for nucleolar accumulation: molecular analysis of the nucleolar targeting mechanism.
    Maeda Y; Hisatake K; Kondo T; Hanada K; Song C; Nishimura...
    The EMBO Journal/11(10)/p.3695-3704, 1992-10
  • The p250 subunit of native TATA box-binding factor TFIID is the cell cycle regulatory protein CCG1.
    Hisatake K; Hasegawa S; Takada R; Nakatani Y; Horikoshi M...
    Nature/362(6416)/p.179-181, 1993-03
  • Functional dissection of TFIIB domains required for TFIIB-TFIID-promoter complex formation and basal transcription activity.
    Hisatake K; Roeder RG; Horikoshi M
    Nature/363(6431)/p.744-747, 1993-06
  • Transcription factor TFIIB sites important for interaction with promoter-bound TFIID.
    Yamashita S; Hisatake K; Kokubo T; Doi K; Roeder RG; Hori...
    Science/261(5120)/p.463-467, 1993-07
  • Regulation of mouse UBF gene by multiple growth-related control elements.
    Nishimura T; Hanada K; Maeda Y; Song C; Hisatake K; Muram...
    Biochemical and Biophysical Research Communications/205(2)/p.1217-1225, 1994-12
  • Identification of an enhancer responsible for a tumor marker gene expression by means of transgenic rats.
    Suzuki T; Imagawa M; Hirabayashi M; Yuki A; Hisatake K; M...
    Cancer Research/55(12)/p.2651-2655, 1995-06
  • Evolutionary conservation of human TATA-binding-polypeptide associated factors TAFII31 and TAFII80 and interactions of TAFII80 with other TAFs and with general transcription factors.
    Hisatake K; Ohta T; Takada R; Guermah M; Horikoshi M; Nak...
    Proceedings of the National Academy of Sciences of the United States of America/92(18)/p.8195-8199, 1995-08
  • Crystal structure of a TFIIB-TBP-TATA-element ternary complex.
    Nikolov DB; Chen H; Halay ED; Usheva AA; Hisatake K; Lee ...
    Nature/377(6545)/p.119-128, 1995-09
  • Unliganded thyroid hormone receptor a can target the TATA-binding protein for transcriptional repression.
    Fondell GD; Brunel F; Hisatake K; Roeder RG
    Molecular and Cellular Biology/16(1)/p.281-287, 1996-01
Books
  • 蛋白質解析法
    久武 幸司
    メディカルサイエンス遺伝子検査学, 2012-02
  • Histone methylation and transcriptional regulation
    福田 綾; 久武幸司
    2007-04
  • Mechanism of histone H3 phosphorylation upon induction of c-fos gene transcription
    嶋田美穂; 中太智義; 福田 綾; 久武幸司
    2007-06
Conference, etc.
  • CARS spectral imaging of iPS cells~Toward visualization of pluripotency~
    米山弘亮; 瀬川尋貴; 西村 健; 福田綾; 久武幸司; 加納英明
    Taiwan International Symposium on Raman Spectroscopy/2014-06
  • Application of cytoplasmic RNA vector to mechanistic analysis of iPSC production and cell differentiation
    Nishimura Ken; T. Kato; D. Ayakawa; L. Oinam; E. Shiomits...
    The 1st National Taiwan University and University of Tsukuba (NTU-UT) Joint Faculty Conference/2014-02--2014-02
  • iPS細胞の多能性可視化に向けたCARS分光イメージング法の開発
    米山弘亮; 瀬川尋貴; 久武幸司; 福田綾; 西村 健; 加納英明
    平成26年度日本分光学会年次講演会/2014-05--2014-05
  • Manipulation of Klf4 expression produces snap shots of iPSC induction
    Nishimura Ken; T. Kato; L. Oinam; T. Narita; M. Ohtaka; A...
    CSHA/ISSCR Joint Meeting on Stem Cells in Science and Medicine/2013-10-14--2013-10-18
  • Manipulation of Klf4 expression produces paused iPSC stalling at distinct intermediate stages of reprogramming
    Nishimura Ken; T. Kato; L. Oinam; D. Ayakawa; M. Ohtaka; ...
    Keystone Symposia “Stem Cells and Reprogramming”/2014-04-06--2014-04-11
  • 初期化が途中で停止した細胞を用いたiPS細胞誘導過程の解析
    西村健; 加藤哲男; Oinam Lalhaba; 塩満鋭美; 成田孝仁; 大高...
    第13回日本再生医療学会総会/2014-03
  • Analysis of the Partially Reprogrammed iPS Cells
    Shiomitsu E; Nishimura K; Kato T; Oinam L; FUKUDA AYA; Ko...
    The 12th NTU-Japan International Student Mini-Symposium on Life Science/2013-12
  • iPS細胞誘導初期におけるメカニズム解析
    加藤哲男; 西村健; 塩満鋭美; 綾川大介; 福田 綾; 久武幸司
    第36回日本分子生物学会年会/2013-12
  • Klf4遺伝子発現量による初期化が途中で停止した細胞の誘導
    西村健; 加藤哲男; Oinam Lalhaba; 塩満鋭美; 成田孝仁; 大高...
    第36回日本分子生物学会年会/2013-12
  • Manipulation of Klf4 expression produces snap shots of iPSC induction
    FUKUDA AYA; Nishimura K; Kato T; Oinam L; Ohtaka M; Nakan...
    CSHAsia 2013 Conference on CSHA/ISSCR Joint Meeting on Stem Cells in Science and Medicine/2013-10
  • Regulated Induction of Partially Reprogrammed iPS Cells by SeVdp Vectors
    Hisatake K; Kato T; Oinam L; Narita T; Ohtaka M; FUKUDA A...
    Cold Spring Harbor Laboratory meeting on “Stem Cell Biology”/2013-09
  • SeVdpベクターを用いたiPS細胞の品質調節メカニズムの解析
    西村健; 成田孝仁; 大高真奈美; 高安聡子; 佐野将之; 福田 綾...
    第12回日本再生医療学会総会/2013-03
  • The RNA-binding protein complexes NF45/90 and NF45/110 activate c-fos transcription in a signal-dependent way
    Fukuda Aya; Nakadai Tomoyoshi; Shimada Miho; Nishimura Ke...
    第35回日本分子生物学会年会/2012-12-11--2012-12-14
  • QUALITY CONTROL OF IPS CELLS BY UNIQUE GENE TRANSFER SYSTEM
    Nishimura Ken; Ohtaka Manami; Takayasu Satoko; Sano Masay...
    International Society of Stem Cell Research 10th Annual Meeting/2012-06-13--2012-06-16
  • RNA工学とウイルス学の融合による簡便かつ高効率なiPS細胞樹立方法の確立
    西村 健; 大高 真奈美; 高安 聡子; 佐野 将之; 久武 幸司; 中...
    第34回日本分子生物学会年会/2011-12-13--2012-12-16
  • Heterogeneous nuclear ribonucleoprotein R (hnRNP R) cooperates with Mediator to enhance transcription reinitiation
    Fukuda Aya; Shimada Miho; Nakadai Tomoyoshi; Nishimura Ke...
    第34回日本分子生物学会年会/2011-12-15
  • Novel Cytoplasmic persistent expression RNA vector developed by combination of RNA biotechnology and virology
    Nishimura Ken; Ohtaka Manami; Sano Masayuki; Hisatake Koj...
    NTU-JST Joint Meeting on RNA and Biofunctions - Asia Studies/2011-11-10--2011-11-12
  • Activation of multiple-round transcriptions by a novel coactivator hnRNP R and Mediator
    Fukuda Aya; Takeuchi Michiyo; Nakadai Tomoyoshi; Shimada ...
    Cold Spring Harbor Laboratory meeting on “Mechanisms of Eukaryotic Transcription/2011-08-31
  • Mechanistic analysis of c-fos transcriptional regulation by a novel coactivator hnRNP R
    Fukuda Aya; Takeuchi Michiyo; Nakadai Tomoyoshi; Shimada ...
    第33回日本分子生物学会年会・第83回日本生化学会合同大会/2010-12-07
  • Mechanistic analyses of transcriptional regulation of the c-fos gene via the NF complexes
    Nakadai Tomoyoshi; Fukuda Aya; Takeuchi Michiyo; Shimada ...
    第33回日本分子生物学会年会・第83回日本生化学会合同大会/2010-12-07
  • Mechanism of c-fos transcriptional regulation by an RNA-binding protein, hnRNP R
    Hisatake Koji; Takeuchi Michiyo; Nakadai Tomoyoshi; Shima...
    Leading Graduate Schools International Conference/2010-11-01
  • Mechanism of c-fos transcriptional regulation by an RNA-binding protein, hnRNP R
    Hisatake Koji; Takauchi Michiyo; Nakadai Tomoyoshi; Shima...
    ASBMB Special Symposia "Transcription Regulation by Chromatin and RNA Polymerase II"/2010-10-02
  • Mechanistic Analysis of c-fos Transcriptional Regulation by a Novel Coactivator hnRNP R
    福田 綾; 竹内 道代; 中太 智義; 嶋田 美穂; 久武 幸司
    9th EMBL Conference "Transcription and Chromatin"/2010-08-29
  • Taking snap shots during iPSC production by SeVdp vectors.
    Nishimura Ken; Kato Tetsuo; Lalhaba Oinam; Narita Takahit...
    International Society of Stem Cell Research 11th Annual Meeting/2013-06-14
  • SeVdpベクターを用いたiPS細胞の品質調節メカニズムの解析
    西村 健; 成田 孝仁; 大高 真奈美; 高安 聡子; 佐野 将之...
    第12回日本再生医療学会総会/2013-03-22
Teaching
2017-04 -- 2017-08Advanced Exercise on Public HealthUniversity of Tsukuba.
2017-10 -- 2018-02Advanced Exercise on Public HealthUniversity of Tsukuba.
2017-10 -- 2018-02Internship IIUniversity of Tsukuba.
2017-04 -- 2017-08Internship IIUniversity of Tsukuba.
2017-04 -- 2017-07Topics in BiochemistryUniversity of Tsukuba.
2017-10 -- 2017-12OncologyUniversity of Tsukuba.
2017-10 -- 2018-02Seminar on Basic Medical SciencesUniversity of Tsukuba.
2017-04 -- 2017-08Seminar on Basic Medical SciencesUniversity of Tsukuba.
2017-07 -- 2017-08Topics in Medical ScienceUniversity of Tsukuba.
2017-07 -- 2017-08BiochemistryUniversity of Tsukuba.
Professional activities
2006 -- (current)THE JAPANESE BIOCHEMICAL SOCIETY評議員

(Last updated: 2017-04-10)