You are here

Hisatake Koji

Affiliation
Faculty of Medicine
Official title
Professor
URL
Email
 
Fax
029-853-3929
Research fields
General medical chemistry
Biomolecular chemistry
Research keywords
Transcription
Gene Expression
Chromatin
Mechanisms of Transcriptional Regulation
iPS Cell
Regenerative Medicine
Research projects
Tcl1を利用したゲノム変異の少ないiPS細胞誘導方法の開発2017 -- 2018久武 幸司Japan Society for the Promotion of Science/Challenging Research (Exploratory)6,370,000Yen
iPS細胞誘導過程でのKlf4による細胞形態と細胞機能のリプログラミング機構2017 -- 2020久武 幸司Japan Society for the Promotion of Science/Grant-in-Aid for Scientific Research(B)17,680,000Yen
iPS細胞誘導の初期段階での転写因子ネットワークの解析2013-04 -- 2016-03Koji HisatakeJapan Society of for the Promotion of Science/Grant-in-Aid for Scientific Research5,200,000Yen
新規コアクチベーターNF複合体の構成因子と制御機構の解析2010-04 -- 2013-03久武幸司Japan Society of for the Promotion of Science/基盤研究(C)4,580,000Yen
リン酸化酵素によるクロマチン修飾の制御機構の解明                2006 -- 2007久武幸司Japan Society of for the Promotion of Science/基盤研究(C)3,890,000Yen
新規コアクチベーターによる転写過程の包括的調節機構の解析            2006 -- 2007久武幸司Ministry of Education, Culture, Sports, Science and Technology, Japan/特定領域研究8,700,000Yen
Career history
1991-04 -- 1994-03ロックフェラー大学 ポストドクトラル フェロー
1994-04 -- 1994-10ロックフェラー大学 ポストドクトラル アソシエート
1994-11 -- 2000-05埼玉医科大学 医学部 講師
2000-06 -- 2005-12埼玉医科大学 医学部 助教授
2006-01 -- 2011-09筑波大学 人間総合科学研究科 教授
2011-10 -- (current)筑波大学 医学医療系 教授
Academic background
-- 1985岡山大学 医学部 医学科Graduated
-- 1991東京大学大学院 医学系研究科 第二基礎医学Completed
Degree
医学博士東京大学
Academic societies
2013-01 -- (current)THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE
2002 -- (current)THE JAPANESE BIOCHEMICAL SOCIETY
1995 -- (current)The Molecular Biology Society of Japan
2003 -- (current)American Society for Biochemistry and Molecular Biology
Articles
  • Live cell imaging of X chromosome reactivation during somatic cell reprogramming
    Tran Thi Hai Yen; Fukuda Aya; Aizawa Shiho; Bui Phuong Li...
    Biochemistry and biophysics reports/15/pp.86-92, 2018-09
  • Promotion of iPSC generation through a metabolic shift by Tcl1
    西村 健; 久武幸司
    医学のあゆみ/265(4)/pp.293-294, 2018-04
  • 新規ベクターシステムを利用した高純度分化細胞単離法
    西村 健; Tran Vo Kieu Nhi; 久武幸司
    PHARMASTAGE/17(10)/pp.11-15, 2018-01
  • Manipulation of KLF4 expression generates iPSCs paused at successive stages of reprogramming
    Ken Nishimura; Tetsuo Kato; Chen Chen; Lalhaba Oinam; Emi...
    Stem Cell Reports, 2014-11
  • Klf4遺伝子発現調節によるiPS細胞誘導中間体の作製
    西村 健; 久武幸司
    医学のあゆみ/255(2)/pp.173-174, 2015-10
  • The RNA Binding Complexes NF45-NF90 and NF45-NF110 Associate Dynamically with the c-fos Gene and Function as Transcriptional Coactivators
    Nakadai Tomoyoshi; Fukuda Aya; Shimada Miho; Nishimura Ke...
    JOURNAL OF BIOLOGICAL CHEMISTRY/290(44)/pp.26832-26845, 2015-10
  • Association of the winged helix motif of the TFIIE alpha subunit of TFIIE with either the TFIIE beta subunit or TFIIB distinguishes its functions in transcription
    Tanaka Aki; Akimoto Yusuke; Kobayashi Satoko; Hisatake Ko...
    GENES TO CELLS/20(3)/pp.203-216, 2015-03
  • The Paired-box Homeodomain Transcription Factor Pax6 Binds to the Upstream Region of the TRAP Gene Promoter and Suppresses Receptor Activator of NF-kappa B Ligand (RANKL)-induced Osteoclast Differentiation
    Kogawa Masakazu; Hisatake Koji; Atkins Gerald J.; Findlay...
    JOURNAL OF BIOLOGICAL CHEMISTRY/288(43)/pp.31299-31312, 2013-10
  • Manipulation of KLF4 expression generates iPSCs paused at successive stages of reprogramming
    Ken Nishimura; Tetsuo Kato; Chen Chen; Lalhaba Oinam; Emi...
    Stem Cell Reports/3(5)/pp.915-929, 2014-11
  • The Paired-box Domain Transcription Factor Pax6 Binds to the Upstream Region of the TRAP Gene Promoter and Suppresses RANKL-induced Osteoclast Differentiation.
    FUKUDA AYA; Kogawa M; Hisatake K; Atkins GJ; Findlay DM; ...
    Journal of Biological Chemistry/288(43)/pp.31299-31312, 2013-10
  • Heterogeneous Nuclear Ribonucleoprotein R Cooperates with Mediator to Facilitate Transcription Reinitiation on the c-Fos Gene
    Fukuda Aya; Shimada Miho; Nakadai Tomoyoshi; Nishimura Ke...
    PLOS ONE/8(8), 2013-08
  • DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation
    Chen Yexi; Yamaguchi Yuki; Tsunego Yuta; Yamamoto Junichi...
    Genes & Development/23(23)/pp.2765-2777, 2009-12
  • Immunoelectron microscopy of adult T cell leukemia-associated antigen by the peroxidase-labeled antibody method.
    Oda T; Watanabe S; Araki Y; Hatsushika M; Sumi H; Nakamur...
    Cellular and Molecular Biology/31(1)/p.49-56, 1985-01
  • Sequence-specific binding of a transcription factor TFID to the promoter region of mouse ribosomal RNA gene.
    Tanaka N; Kato H; Ishikawa Y; Hisatake K; Tashiro K; Komi...
    The Journal of Biological Chemistry/256(23)/p.12836-13842, 1990-08
  • Structure of the core promoter of human and mouse ribosomal RNA gene: Asymmetry of species-specific transcription.
    Ishikawa Y; Safrany G; Hisatake K; Tanaka N; Maeda Y; Kat...
    Journal of Molecular Biology/218(1)/p.55-67, 1991-03
  • Cloning and structural analysis of cDNA and its gene for mouse transcription factor UBF.
    Hisatake K; Nishimura T; Maeda Y; Hanada K; Song C; Muram...
    Nucleic Acids Research/19(17)/p.4631 4637, 1991-09
  • Sequence of general transcription factor TFIIB and relationships to other initiation factors.
    Malik S; Hisatake K; Sumimoto H; Horikoshi M; Roeder RG
    Proceedings of the National Academy of Sciences of the United States of America/88(21)/p.9553-9557, 1991-11
  • Conserved structural motifs between Xenopus and human TFIIB.
    Hisatake K; Malik S; Roeder RG; Horikoshi M
    Nucleic Acids Research/19(23)/p.6639, 1991-12
  • Isolation and characterization of a cDNA encoding Drosophila transcription factor TFIIB.
    Yamashita S; Wada K; Horikoshi M; Gong D-W; Kokubo T; His...
    Proceedings of the National Academy of Sciences of the United States of America/89(7)/p.2839-2843, 1992-04
  • Mouse rRNA transcription factor mUBF requires both HMG-box1 and an acidic tail for nucleolar accumulation: molecular analysis of the nucleolar targeting mechanism.
    Maeda Y; Hisatake K; Kondo T; Hanada K; Song C; Nishimura...
    The EMBO Journal/11(10)/p.3695-3704, 1992-10
  • The p250 subunit of native TATA box-binding factor TFIID is the cell cycle regulatory protein CCG1.
    Hisatake K; Hasegawa S; Takada R; Nakatani Y; Horikoshi M...
    Nature/362(6416)/p.179-181, 1993-03
  • Functional dissection of TFIIB domains required for TFIIB-TFIID-promoter complex formation and basal transcription activity.
    Hisatake K; Roeder RG; Horikoshi M
    Nature/363(6431)/p.744-747, 1993-06
  • Transcription factor TFIIB sites important for interaction with promoter-bound TFIID.
    Yamashita S; Hisatake K; Kokubo T; Doi K; Roeder RG; Hori...
    Science/261(5120)/p.463-467, 1993-07
  • Regulation of mouse UBF gene by multiple growth-related control elements.
    Nishimura T; Hanada K; Maeda Y; Song C; Hisatake K; Muram...
    Biochemical and Biophysical Research Communications/205(2)/p.1217-1225, 1994-12
  • Identification of an enhancer responsible for a tumor marker gene expression by means of transgenic rats.
    Suzuki T; Imagawa M; Hirabayashi M; Yuki A; Hisatake K; M...
    Cancer Research/55(12)/p.2651-2655, 1995-06
Books
  • 蛋白質解析法
    久武 幸司
    メディカルサイエンス遺伝子検査学, 2012-02
  • Histone methylation and transcriptional regulation
    福田 綾; 久武幸司
    biochemistry The Japanese Biochemical Society, 2007-04
  • Mechanism of histone H3 phosphorylation upon induction of c-fos gene transcription
    嶋田美穂; 中太智義; 福田 綾; 久武幸司
    Jikken Igaku, vol.25, No.10 Yodosha, 2007-06
Conference, etc.
  • Klf4による多能性獲得はTcl1を介した代謝変化により起きる
    相澤 志穂; 西村 健; Nugroho Fransiska Liliani; 櫻木 佑...
    第17回日本再生医療学会総会/2018-03-22--2018-03-23
  • X染色体の再活性化をモデルとしたエピジェネティクス転写制御機構の解明
    相澤 志穂; Bui Phuong Linh; 西村 健; 久武幸司
    超異分野学会2018/2018-03-02--2018-03-03
  • Tcl1による代謝リプログラミングはiPS細胞の多能性を向上させる
    西村 健; 相澤 志穂; 櫻木 佑太; 林 洋平; 福田 綾; 久武...
    ConBio2017/2017-12-06--2017-12-09
  • iPS細胞誘導におけるX染色体再活性化機構の解析
    相澤 志穂; Bui Phuong Linh; 西村 健; 久武幸司
    ConBio2017/2017-12-06--2017-12-09
  • Mechanistic analysis of the initiation of retroviral gene silencing by reprogramming factors
    Linh Bui Phuong; Nishimura Ken; 久武幸司
    ConBio2017/2017-12-06--2017-12-09
  • Mechanistic analysis of the initiation of retroviral gene silencing by reprogramming factors
    Linh Bui Phuong; Nishimura Ken; Hisatake Koji
    The 6th Human Biology Symposium/2017-09-27--2017-09-27
  • Analysis of X Chromosome Reactivation During Reprogramming
    FUKUDA AYA; Yen Tran Thi Hai; YOHEI HAYASHI; KEN NISHIMUR...
    Tsukuba Global Science Week 2017/2017-09
  • UCP1発現脂肪細胞の非侵襲イメージング法の作製
    福田 綾; 本多志帆; 水野聖哉; 杉山文博; 岡松優子; 斉藤昌之...
    第40回日本分子生物学会年会/2017-12
  • Production of a non-invasive monitoring system of Uncoupling protein 1-expressing adipocytes
    FUKUDA AYA; HONDA SHIHO; SEIYA MIZUNO; FUMIHIRO SUGIYAMA;...
    Tsukuba Global Science Week 2017/2017-09
  • CARS spectral imaging of iPS cells~Toward visualization of pluripotency~
    米山弘亮; 瀬川尋貴; 西村 健; 福田綾; 久武幸司; 加納英明
    Taiwan International Symposium on Raman Spectroscopy/2014-06
  • Application of cytoplasmic RNA vector to mechanistic analysis of iPSC production and cell differentiation
    Nishimura Ken; T. Kato; D. Ayakawa; L. Oinam; E. Shiomits...
    The 1st National Taiwan University and University of Tsukuba (NTU-UT) Joint Faculty Conference/2014-02--2014-02
  • iPS細胞の多能性可視化に向けたCARS分光イメージング法の開発
    米山弘亮; 瀬川尋貴; 久武幸司; 福田綾; 西村 健; 加納英明
    平成26年度日本分光学会年次講演会/2014-05--2014-05
  • Manipulation of Klf4 expression produces snap shots of iPSC induction
    Nishimura Ken; T. Kato; L. Oinam; T. Narita; M. Ohtaka; A...
    CSHA/ISSCR Joint Meeting on Stem Cells in Science and Medicine/2013-10-14--2013-10-18
  • Manipulation of Klf4 expression produces paused iPSC stalling at distinct intermediate stages of reprogramming
    Nishimura Ken; T. Kato; L. Oinam; D. Ayakawa; M. Ohtaka; ...
    Keystone Symposia “Stem Cells and Reprogramming”/2014-04-06--2014-04-11
  • 初期化が途中で停止した細胞を用いたiPS細胞誘導過程の解析
    西村健; 加藤哲男; Oinam Lalhaba; 塩満鋭美; 成田孝仁; 大高...
    第13回日本再生医療学会総会/2014-03
  • Analysis of the Partially Reprogrammed iPS Cells
    Shiomitsu E; Nishimura K; Kato T; Oinam L; FUKUDA AYA; Ko...
    The 12th NTU-Japan International Student Mini-Symposium on Life Science/2013-12
  • iPS細胞誘導初期におけるメカニズム解析
    加藤哲男; 西村健; 塩満鋭美; 綾川大介; 福田 綾; 久武幸司
    第36回日本分子生物学会年会/2013-12
  • Klf4遺伝子発現量による初期化が途中で停止した細胞の誘導
    西村健; 加藤哲男; Oinam Lalhaba; 塩満鋭美; 成田孝仁; 大高...
    第36回日本分子生物学会年会/2013-12
  • Manipulation of Klf4 expression produces snap shots of iPSC induction
    FUKUDA AYA; Nishimura K; Kato T; Oinam L; Ohtaka M; Nakan...
    CSHAsia 2013 Conference on CSHA/ISSCR Joint Meeting on Stem Cells in Science and Medicine/2013-10
  • Regulated Induction of Partially Reprogrammed iPS Cells by SeVdp Vectors
    Hisatake K; Kato T; Oinam L; Narita T; Ohtaka M; FUKUDA A...
    Cold Spring Harbor Laboratory meeting on “Stem Cell Biology”/2013-09
  • SeVdpベクターを用いたiPS細胞の品質調節メカニズムの解析
    西村健; 成田孝仁; 大高真奈美; 高安聡子; 佐野将之; 福田 綾...
    第12回日本再生医療学会総会/2013-03
  • The RNA-binding protein complexes NF45/90 and NF45/110 activate c-fos transcription in a signal-dependent way
    Fukuda Aya; Nakadai Tomoyoshi; Shimada Miho; Nishimura Ke...
    第35回日本分子生物学会年会/2012-12-11--2012-12-14
  • QUALITY CONTROL OF IPS CELLS BY UNIQUE GENE TRANSFER SYSTEM
    Nishimura Ken; Ohtaka Manami; Takayasu Satoko; Sano Masay...
    International Society of Stem Cell Research 10th Annual Meeting/2012-06-13--2012-06-16
  • RNA工学とウイルス学の融合による簡便かつ高効率なiPS細胞樹立方法の確立
    西村 健; 大高 真奈美; 高安 聡子; 佐野 将之; 久武 幸司; 中...
    第34回日本分子生物学会年会/2011-12-13--2012-12-16
  • Heterogeneous nuclear ribonucleoprotein R (hnRNP R) cooperates with Mediator to enhance transcription reinitiation
    Fukuda Aya; Shimada Miho; Nakadai Tomoyoshi; Nishimura Ke...
    第34回日本分子生物学会年会/2011-12-15
Intellectural property rights
  • 遺伝子改変非ヒト動物及びその使用
    福田 綾; HONDA SHIHO; KOJI HISATAKE
Teaching
2018-04 -- 2018-07Introduction to Medical ScienceUniversity of Tsukuba.
2018-04 -- 2018-07Topics in BiochemistryUniversity of Tsukuba.
2018-10 -- 2019-02Dissertation in Medical SciencesUniversity of Tsukuba.
2018-10 -- 2019-02Internship IUniversity of Tsukuba.
2018-10 -- 2019-02Internship IIUniversity of Tsukuba.
2018-10 -- 2019-02Seminar on Basic Medical SciencesUniversity of Tsukuba.
2018-10 -- 2019-02Advanced Exercise on Public HealthUniversity of Tsukuba.
2018-04 -- 2018-08Dissertation in Medical SciencesUniversity of Tsukuba.
2018-04 -- 2018-08Internship IUniversity of Tsukuba.
2018-04 -- 2018-08Internship IIUniversity of Tsukuba.
Professional activities
2006 -- (current)THE JAPANESE BIOCHEMICAL SOCIETY評議員

(Last updated: 2017-04-10)