Ohtsu Iwao

Researcher's full information

Refereed academic journal/Refereed international conference paper

• A mutation in dnaK causes stabilization of the heat shock sigma factor σ32, accumulation of heat shock proteins and increase in toluene-resistance in Pseudomonas putida.
  Ohtsu Iwao
  Environ Microbiol./13(8)/pp.2007-2017, 2011-08
  PubMed
• The L-cysteine/L-cystine shuttle system provides reducing equivalents to the periplasm in Escherichia coli.
  Ohtsu Iwao
  The Journal of Biological Chemistry/285(23)/pp.17479-17487, 2010-06
  PubMed
• Identification of zinc-responsive proteins in the roots of Arabidopsis thaliana using a highly improved method of two-dimensional electrophoresis.
  Ohtsu Iwao
  Plant Cell Physiol./50(12)/pp.2234-2239, 2009-12
  PubMed
• A functional analysis of the yeast ubiquitin ligase Rsp5: the involvement of the ubiquitin-conjugating enzyme Ubc4 and poly-ubiquitination in ethanol-induced down-regulation of targeted proteins.
  Ohtsu Iwao
  Biosci Biotechnol Biochem., 2009-10
  PubMed
• The yeast ubiquitin ligase Rsp5 downregulates the alpha subunit of nascent polypeptide-associated complex Egd2 under stress conditions.
  Ohtsu Iwao
  FEBS J., 2009-09
  PubMed
• The outer membrane TolC is involved in cysteine tolerance and overproduction in Escherichia coli.
  Ohtsu Iwao
  Appl Microbiol Biotechnol./81(5)/pp.903-913, 2009-01
  PubMed
• OxyR is involved in the expression of thioredoxin reductase TrxB in Pseudomonas putida.
  Ohtsu Iwao
  FEMS Microbiol Lett./282(2)/pp.138-145, 2008-12
  PubMed
• alpha-ketoglutaric semialdehyde dehydrogenase isozymes involved in metabolic pathways of D-glucarate, D-galactarate, and hydroxy-L-proline. Molecular and metabolic convergent evolution.
  Ohtsu Iwao
  The Journal of Biological Chemistry/282(9)/pp.6685-6695, 2007-03
  PubMed
• Transcriptional analysis of the Escherichia coli mreBCD genes responsible for morphogenesis and chromosome segregation.
  Ohtsu Iwao
  Biosci Biotechnol Biochem./70(11)/pp.2712-2719, 2006-11
  PubMed
• Direct matrix-assisted laser desorption/ionization time-of-flight mass spectrometric identification of proteins on membrane detected by Western blotting and lectin blotting.
  Ohtsu Iwao
  J Proteome Res./4(4)/pp.1391-1396, 2005-08
  PubMed
• Direct MS/MS analysis of proteins blotted on membranes by a matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight tandem mass spectrometer.
  Ohtsu Iwao
  J Proteome Res./4(3)/pp.743-747, 2005-06
  PubMed
• Convenient and sensitive evaluation of a superoxide anion-generating reagent methyl viologen by Escherichia coli harboring a soxS'::gfp reporter plasmid.
  Ohtsu Iwao
  Biosci Biotechnol Biochem./68(12)/pp.2637-2639, 2004-12
  PubMed
• Transcriptional analysis of the ostA/imp gene involved in organic solvent sensitivity in Escherichia coli.
  Ohtsu Iwao
  Biosci Biotechnol Biochem./94(6)/pp.542-548, 2004-02
  PubMed
• n-Hexane sensitivity of Escherichia coli due to low expression of imp/ostA encoding an 87 kDa minor protein associated with the outer membrane.
  Ohtsu Iwao
  Microbiology./149(Pt 5)/pp.1265-1273, 2003-03
  PubMed
• Random mutagenesis into the conserved Gly154 of subtilisin E: isolation and characterization of the revertant enzymes.
  Ohtsu Iwao
  Protein Eng./11(12)/pp.1205-1210, 1998-12
  PubMed
• Construction of novel subtilisin E with high specificity, activity and productivity through multiple amino acid substitutions.
  Ohtsu Iwao
  Protein Eng./10(9)/pp.985-989, 1997-09
  PubMed
• Restriction of substrate specificity of subtilisin E by introduction of a side chain into a conserved glycine residue.
  Ohtsu Iwao
  FEBS Lett./395(2-3)/pp.127-132, 1996-10
  PubMed