MIZUTANI Eiji

Affiliation
Institute of Medicine
Official title
Associate Professor
Research keywords
clone
embryology
クローン家畜
発生工学
Research projects
Control of mouse individuals by chromosomes from other animal species2025 -- 2027水谷 英二Japan Society for the Promotion of Science/Grant-in-Aid for Challenging Research (Exploratory)6,370,000Yen
in vivo development of non-human primate blastoids2023-04 -- 2026-03MASAKI HidekiJapan Society for the Promotion of Science/Grant-in-Aid for Scientific Research (B)3,000,000Yen
生体を用いた腫瘍抗原特異的ヒトT細胞受容体スクリーニング系の開発と利用2022-09 -- 2025-03Teruhiko SUZUKIJapan Agency for Medical Research and Development (AMED)/次世代がん医療加速化研究事業 (分担者)7,800,000Yen
Development of a mouse model of human trisomy by chromosome transfer2020-04 -- 2023-03MIZUTANI EijiJapan Society for the Promotion of Science/Grant-in-Aid for Scientific Research(B)17,680,000Yen
Development of in vitro chimera assay system utilizing cynomolgus monkey embryos2020-04 -- 2023-03Hideki MASAKIJapan Society for the Promotion of Science/Grant-in-Aid for Scientific Research (B)17,810,000Yen
Generation of human pancreas in xenogeneic animal2020-04 -- 2023-03YAMAGUCHI TomoyukiJapan Society for the Promotion of Science/Grant-in-Aid for Scientific Research (B)3,000,000Yen
ヒト/マウス人工染色体を用いたゲノムライティングと応用2019-04 -- 2023-03Yasuhiro KAZUKIJapan Science and Technology Agency (JST)/JST戦略的創造研究推進事業（CREST）15,000,000Yen
Feasibility Study for the generation of live cell from extinct or endangered animal2016-04 -- 2019-03若山照彦Japan Society for the Promotion of Science/Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)40,560,000Yen
Analysis of individual chromosome dynamics in living embryo and establishment of chromosome manipulation technique2015-04 -- 2018-03水谷英二Japan Society for the Promotion of Science/Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)17,030,000Yen
Searching for prerequisites for full term development of cloned embryos based on the live cell imaging analysis2012-04 -- 2015-03水谷英二Japan Society for the Promotion of Science (JSPS)/Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)4,550,000Yen
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Career history
2020-05 -- (current)University of TsukubaFaculty of Medicine准教授
2018-04 -- 2020-03Institute of Medical Science, University of TokyoDivision of Stem Cell Therapyproject associate professor
2016-07 -- 2018-03Institute of Medical Science, University of TokyoDivision of Stem Cell Therapyproject researcher
2012-04 -- 2016-06University of YamanashiFaculty of Life and Environmental Sciencesassistant professor
2011-04 -- 2012-03RIKEN BRCBioresource Engineering Divisionresearcher
2009-07 -- 2011-03National Institute of Livestock and Grassland ScienceReproductive Biology and Technology Research Teamproject researcher
2006-04 -- 2009-06RIKEN CDBLaboratory for Genomic Reprogrammingresearcher
2020-04 -- (current)東京大学医科学研究所幹細胞治療部門特任研究員
Academic background
2003-04 -- 2006-03Tohoku University 農学研究科
2001-04 -- 2003-03Tohoku University 農学研究科
-- 2003-01Tohoku University Graduate School, Division of Agriculture
1997-04 -- 2001-03Tohoku University Faculty of Agriculture
-- 2001-01Tohoku University Faculty of Agriculture
Degree
2006-3博士（農学）東北大学
Academic societies
-- (current)JAPAN SOCIETY FOR OVA RESEARCH
-- (current)THE MOLECULAR BIOLOGY SOCIETY OF JAPAN
-- (current)The Japanese Society of Animal Reproduction
-- (current)Society for the Study of Reproduction
-- (current)THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT
Articles
  • Highly efficient and low-mosaicism piggyBac transgenesis platform for rapid founder phenotyping
    Okamura Eiichi; Matsumoto Shoma; Mizutani Eiji; Murata...
    iScience/29(7), 2026-07-17
    PubMed
  • Skin graft with dermis and appendages generated in vivo by cell competition
    Nagano Hisato; Mizuno Naoaki; Sato Hideyuki; Mizutani ...
    NATURE COMMUNICATIONS/15(1), 2024-04-29
  • Functional calcium-responsive parathyroid glands generated using single-step blastocyst complementation
    Kano Mayuko; Mizuno Naoaki; Sato Hideyuki; Kimura Tak...
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA/120(28), 2023-07-11
    PubMed
  • Removal of sperm tail using trypsin and pre-activation of oocyte facilitates intracytoplasmic sperm injection in mice and rats
    Torikai Kohei; Shimizu Kazuma; Nagatomo Hiroaki; Kasai...
    The Journal of reproduction and development/69(1)/pp.48-52, 2023-02-08
    PubMed
  • Chemically defined cytokine-free expansion of human haematopoietic stem cells
    Sakurai Masatoshi; Ishitsuka Kantaro; Ito Ryoji; Wilki...
    NATURE/615(7950)/pp.127-133, 2023-02
    PubMed
  • Xenotransplantation and interspecies organogenesis: current status and issues
    Kano Mayuko; Mizutani Eiji; Homma Shota; Masaki Hidek...
    FRONTIERS IN ENDOCRINOLOGY/13, 2022-08
  • Parthenogenetic activation and subsequent development of rat oocytes in vitro
    JY Jiang S Mizuno E Mizutani H Sasada E Sato
    Molecular Reproduction and Development/61(1)/pp.120-125, 2002-01
    PubMed
  • Nuclear transfer in rats using an established embryonic cell line and cumulus cells
    JY Jiang S Mizuno E Mizutani K Miyoshi N Kimura H Sa...
    JOURNAL OF REPRODUCTION AND DEVELOPMENT/48(5)/pp.505-511, 2002-10
    CiNii
  • Different methylation states of the zygotic paternal genome after ICSI and ROSI.
    S Kishigami VT Nguyen T Hikichi H Ohta S Wakayama E ...
    BIOLOGY OF REPRODUCTION/pp.100-100, 2004-01
  • Effects of treatments of donor somatic cells on the efficiency of mouse cloning.
    VT Nguyen S Wakayama S Kishigami T Hikichi H Ohta E ...
    BIOLOGY OF REPRODUCTION/pp.239-239, 2004-01
  • Normality of nuclear transfer embryonic stem cell lines derived from adult somatic cells.
    S Wakayama S Senda T Hikichi S Kishigami E Mizutani ...
    BIOLOGY OF REPRODUCTION/pp.90-90, 2005-01
  • Generation of progeny from embryonic stem cells by microinsemination of male germ cells from chimeric mice
    Mizutani E; Ohta H; Kishigami S; Thuan N Van; Hikichi T; ...
    In Challenge of Animal Industry for the Wellbeing of Mankind, XIIth AAAP Animal Science Congress 2006/pp.4-8, 2006-01
  • Supplementation of a cloned embryo with ES/ntES cells can increase the cell number of the blastocyst but not rescue full term development.
    Eiji Mizutani Satoshi Kishigami Nguyen Van Thuan Hiros...
    BIOLOGY OF REPRODUCTION/pp.180-180, 2006-01
  • Full-term development of mouse embryos produced from reconstructed giant oocytes by intracytoplasmic sperm head injection
    Wakayama S.; Kishigami S.; T. Nguyen V.; Ohta H.; Hikichi...
    REPRODUCTION FERTILITY AND DEVELOPMENT/19(1)/pp.305-305, 2007-01
  • Cytoplasm of a germinal vesicle oocyte can promote somatic nuclear reprogramming in mice
    T. Bui H.; Van Thuan N.; Kishigami S.; Wakayama S.; Hikic...
    REPRODUCTION FERTILITY AND DEVELOPMENT/19(1)/pp.132-132, 2007-01
  • Success of mouse cloning from an outbred strain by trichostatin A treatment after somatic nuclear transfer
    Kishigami S.; Bui H.; Wakayama S.; Van Thuan N.; Hikichi ...
    REPRODUCTION FERTILITY AND DEVELOPMENT/19(1)/pp.145-145, 2007-01
  • Observation of chromosome decondensation, histone H3 modification, and HP1 protein in mouse cloned embryos following inhibition of histone deacetylations and cyclin-dependent kinase
    Van Thuan N.; Hong-Thuy B.; Wakayama S.; Kishigami S.; Oh...
    REPRODUCTION FERTILITY AND DEVELOPMENT/19(1)/pp.164-165, 2007-01
  • Successful establishment of pluripotent ntES cell lines from aged mice
    Mizutani E.; Kishigami S.; V. Thuan N.; Ohta H.; Hikichi ...
    REPRODUCTION FERTILITY AND DEVELOPMENT/19(1)/pp.151-151, 2007-01
  • Successfully producing cloned mice from somatic cells of aged mice via established ntES cell lines
    Mizutani E.; Ono T.; Chong L.; Wakayama T.
    REPRODUCTION FERTILITY AND DEVELOPMENT/20(1)/pp.104-104, 2008-01
  • Full-term development using the high alkali treating spermatozoa before ICSI
    Chong Li Eiji Mizutani Tetsuo Ono Teruhiko Wakayama
    BIOLOGY OF REPRODUCTION/pp.134-134, 2008-01
  • An Improved ICSI-Mediated Transgenic Method Using NaOH-Treated Spermatozoa
    Chong Li Eiji Mizutani Tetsuo Ono Teruhiko Wakayama
    BIOLOGY OF REPRODUCTION/pp.188-188, 2009-01
  • Production of transgenic mice efficiently using highly alkaline treated spermatozoa before ICSI
    Chong Li Eiji Mizutani Tetsuo Ono Teruhiko Wakayama
    TRANSGENIC RESEARCH/19(2)/pp.337-337, 2010-04
  • EFFECT OF TREATMENT OF BOVINE DONOR CELLS WITH MOUSE EMBRYONIC STEM CELL EXTRACT ON THE DEVELOPMENT OF EMBRYOS AFTER NUCLEAR TRANSFER
    Akagi S.; Mizutani E.; Inaba Y.; Kaneda M.; Somfai T.; Ha...
    REPRODUCTION FERTILITY AND DEVELOPMENT/23(1)/pp.119-119, 2011-01
  • THE EFFECT OF SOURCE AND IN VITRO MATURATION ON THE ABUNDANCE OF MATERNAL mRNA OF SELECTED GENES IN FOLLICULAR BOVINE OOCYTES AND THEIR INFLUENCE ON IN VITRO DEVELOPMENT
    Somfai T.; Imai K.; Kaneda M.; Akagi S.; Haraguchi S.; Wa...
    REPRODUCTION FERTILITY AND DEVELOPMENT/23(1)/pp.199-199, 2011-01
  • PRODUCTION OF NORMAL MICE USING LONG-TERM PRESERVED MOUSE SPERMATOZOA WITHOUT FREEZING
    Li C.; Mizutani E.; Ono T.; Wakayama T.
    REPRODUCTION FERTILITY AND DEVELOPMENT/23(1)/pp.241-241, 2011-01
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Books
  • iPS細胞/ES細胞の生物学【Part1:多能性の誘導】核移植によるリプログラミング機構
    水谷英二 若山清香 若山照彦
    生物の科学 遺伝/pp.20-25, 2014-01
  • 細胞核の初期化メカニズム 多能性・全能性獲得のナゾに迫る 03 ES細胞の樹立からみた「核移植と核のリプログラミング」
    若山清香 水谷英二 若山照彦
    メディカルバイオ/pp.22-27, 2009-09
  • 特集 “遺伝子治療と再生医療” クローン技術の発展と現状
    水谷英二 若山照彦
    腎と透析/pp.459-463, 2005-09
  • ブタの心臓異種移植成功
    加納麻弓子、水谷 英二、中内啓光
    日経サイエンス　/日経サイエンス社/pp.66-69, 2022-04
Conference, etc.
  • In vivo generation of functional adrenal glands from mouse pluripotent stem cells
    水谷 英二
    The 22nd International Congress of Endocrinology and the 99th Annual Congress of the Japan Endocrine Society/2026-6-2--2026-6-6
  • Generation of a mouse model of multiple endocrine neoplasia type1 through developmental and genetic engineering approaches
    水谷 英二
    The 22nd International Congress of Endocrinology and the 99th Annual Congress of the Japan Endocrine Society/2026-6-2--2026-6-6
  • 動物体内環境を利用したマウス多能性幹細胞からの副腎作製
    水谷 英二
    第98回日本内分泌学会学術集会/2025-6-5--2025-6-7
  • Complete humanization of MHC genes in mouse
    水谷 英二
    日本免疫学会学術集会/2025-12-10--2025-12-12
  • Complete humanization of MHC genes in mouse
    水谷 英二
    日本免疫学会学術集会/2025-12-10--2025-12-12
  • Complete humanization of MHC region in mouse
    水谷 英二
    日本免疫学会学術集会/2024-12-03--2024-12-05
  • ミクロセル直接注入によるトランスクロモソミックマウス作出
    水谷 英二
    第46回　日本分子生物学会年会/2023-12-06--2023-12-08
  • ラット細胞質内精子注入法の簡便化の試み
    水谷英二 笠井真理子 加藤めぐみ 中内啓光
    日本生化学会大会(Web)/2017-01
  • マウス2細胞期胚において分配異常を起こした染色体の回収と同定の試み
    柴崎郁江 鎌田裕子 鳥飼昂平 長友啓明 水谷英二 若山照彦
    Journal of Reproduction and Development/2016-09
  • 尿中の体細胞からのクローンマウス作出および核移植胚由来ES細胞株の樹立
    水谷英二 鳥飼昂平 若山清香 長友啓明 大日向康秀 岸上哲士 若山照彦
    Journal of Reproduction and Development/2016-09
  • 国際宇宙ステーションで長期保存したマウス精子からの産仔作出(Space Pup)
    若山清香 鎌田裕子 山中香織 鈴木ひろみ 嶋津徹 嶋津徹 幸田尚 笠原春夫 長田郁子 水谷英二 ...
    日本宇宙航空環境医学会大会プログラム・予稿集/2016-01
  • ntES細胞の点突然変異解析
    砂山美里 荒木良子 藤森(法喜)ゆう子 水谷英二 若山清香 長友啓明 中村美樹 若山照彦 安倍真澄
    日本分子生物学会年会プログラム・要旨集(Web)/2016-01
  • 連続核移植により未受精卵の細胞質に長時間浸したドナー核の発生能について
    若山清香 長友啓明 大日向康秀 水谷英二 若山照彦
    J Reprod Dev/2015-09
  • 胚盤胞注入によって胎盤を置換し得る寄与率を有する新規栄養膜幹細胞の樹立
    大日向康秀 長友啓明 水谷英二 若山照彦
    J Reprod Dev/2015-09
  • 体細胞クローンマウス精子のDNAメチル化異常の検出
    小池佐 神長祐子 若井拓也 若井拓也 水谷英二 小林久人 若山清香 坂下陽彦 三浦史仁 伊藤隆司...
    J Reprod Dev/2015-09
  • 精子の鞭毛除去によるICSI技術の簡便化の試み
    鳥飼昂平 清水和真 鎌田裕子 柴崎郁江 長友啓明 若山清香 水谷英二 若山照彦
    J Reprod Dev/2015-09
  • 前核注入法とICSIを用いたCRISPR/Cas9によるゲノム編集効率について
    長友啓明 柴崎郁江 水谷英二 若山照彦
    J Reprod Dev/2015-09
  • マウス簡易凍結精子融解後の時間経過による発生能低下とその改善
    芦澤俊行 水谷英二 若山照彦
    J Mamm Ova Res/2015-04
  • 鞭毛が乖離した精子を用いたICSI胚の発生率の検討
    清水和真; 水谷英二; 若山照彦
    J Mamm Ova Res/2015-04
  • 化学的定義条件における新規栄養膜幹細胞の樹立
    大日向康秀; 長友啓明; 水谷英二; 若山照彦
    日本生化学会大会(Web)/2015-01
  • Whole genome sequencingによるiPS細胞と核移植ES細胞ゲノム内点突然変異比較
    藤森(法喜)ゆう子 荒木良子 砂山美里 水谷英二 若山清香 長友啓明 笠間康次 中村美樹 若山照彦...
    日本生化学会大会(Web)/2015-01
  • 体細胞クローンマウス精子のDNAメチレーションエラー
    河野友宏 若井拓哉 水谷英二 小林久人 若山清香 坂下陽彦 三浦史仁 伊藤隆司
    J Reprod Dev/2014-08
  • 胎仔期由来前精原細胞核移植クローン胚を用いたマウス父性インプリント確立に関する解析
    上村 悟氏 平澤 竜太郎 松本 和也 井上 貴美子 越後貫 成美 的場 章悟 水谷 英二 及川 真実...
    J Mamm Ova Res/2014-04
  • 体細胞クローンマウス精子のDNAメチル化リプログラミング
    若井拓哉; 水谷英二; 小林久人; 若山清香; 坂下陽彦; 伊藤隆司; 三浦史仁; 河野友宏
    日本分子生物学会年会プログラム・要旨集(Web)/2014-01
  • 無限クローンは可能か
    若山照彦 幸田尚 小保方晴子 野老美紀子 リ チョン 寺下愉加里 水谷英二 グェンバン トン 岸上...
    J Reprod Dev/2013-08
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Intellectural property rights
  • ヒト染色体の分散方法、単離方法、およびその動物胚 への移植方法
    水谷 英二
  • トランスジェニック非ヒト動物の作成方法
    水谷 英二; 太田浩; 若山照彦
Teaching
2025-07 -- 2025-08Embryo Manipulation and Animal ExperimentsUniversity of Tsukuba.
2025-04 -- 2025-06Introduction to Medical ScienceUniversity of Tsukuba.
2025-04 -- 2025-08English Communication for Medical Sciences IIUniversity of Tsukuba.
2025-10 -- 2026-02Lecture and Discussion in Human Medical Biology IIUniversity of Tsukuba.
2025-04 -- 2025-08Lecture and Discussion in Human Medical Biology IUniversity of Tsukuba.
2025-10 -- 2026-02Practice in Human Medical Biology IIUniversity of Tsukuba.
2025-04 -- 2025-08Practice in Human Medical Biology IUniversity of Tsukuba.
2025-10 -- 2026-02Seminar in Human Medical Biology IIUniversity of Tsukuba.
2025-04 -- 2025-08Seminar in Human Medical Biology IUniversity of Tsukuba.
2024-11 -- 2025-02Research SeminarUniversity of Tsukuba.
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(Last updated: 2026-08-07)