HIRAKAWA Hidehiko
- Affiliation
- Institute of Life and Environmental Sciences
- Official title
- Associate Professor
- ORCID
- 0000-0003-0623-8573
- Sex
- Male
- KAKEN ID
- 90451799
- URL
- /9:C2<2H2]9:569:<@]86Tc_F]EDF<F32]24];A
- Research fields
Biofunction/Bioprocess - Research keywords
Cytochrome P450 Protein engineering Enzyme engineering - Research projects
ホモ多量体タンパク質を単量体タンパク質として扱うためのツールの開発 2024-04 -- 2028-03 平川 秀彦 Japan Society for the Promotion of Science/Grant-in-Aid for Scientific Research (C) 4,550,000Yen ゲル状多酵素複合体のサイズ制御 2018-12 -- 2021-03 平川 秀彦 University of Tsukuba/研究基盤支援プログラム(Sタイプ) 3,000,000Yen Discovery of specific redox systems for bacterial cytochrome P450s 2018-04 -- 2022-03 Hidehiko Hirakawa Japan Society for the Promotion of Science/Grant-in-Aid for Scientific Research (C) 4,290,000Yen 水溶性シトクロムP450の触媒活性に必須な電子伝達系の探索法の開発 2016-04 -- 2017-03 Hidehiko Hirakawa ホクト生物科学振興財団/研究奨励金 1,000,000Yen Sequential enzyme complex formation on DNA 2014-04 -- 2016-03 Hidehiko Hirakawa 日本学術振興会/Grant-in-Aid for Challenging Exploratory Research 4,030,000Yen 自己集積する足場タンパク質を利用した人工セルロソーム 2012-10 -- 2013-09 Hidehiko Hirakawa 積水化学/自然に学ぶものづくり研究助成プログラム 1,000,000Yen Staphylococcus aureus由来ソルテースA変異体を利用した細胞内蛋白質融合 2012-04 -- 2013-03 Hidehiko Hirakawa JBA/化学素材研究開発振興財団記念基金「グラント」研究奨励金 500,000Yen 足場タンパク質を利用したシトクロムP450の自己組織的な固定化 2012-04 -- 2013-03 Hidehiko Hirakawa 旭硝子財団/平成24年度自然科学系「研究奨励」プログラム 2,000,000Yen 細胞内における選択的なタンパク質連結法の開発 2011-04 -- 2012-03 Hidehiko Hirakawa みずほ学術振興財団/第54回工学研究助成 2,000,000Yen Complete Self-sufficient P450 with Cofactor Regeneration Domain 2011-01 -- 2011-12 Hidehiko Hirakawa ノボザイムズ ジャパン/研究ファンド2011 1,000,000Yen more... - Career history
2010-11 -- 2018-02 The University of TokyoDepartment of Chemistry and Biotechnology, School of EngineeringAssistant Professor 2010-04 -- 2010-11 The University of TokyoDepartment of Chemistry and Biotechnology, School of EngineeringProject Assistant Professor 2009-01 -- 2010-03 The University of TokyoSchool of EngineeringProject Assistant Professor 2008-03 -- 2009-01 The University of TokyoSchool of EngineeringProject postdoctoral fellow 2007-04 -- 2008-03 The University of TokyoCenter for NanoBio IntegrationProject postdoctoral fellow 2005-04 -- 2007-03 The University of KansasHiguchi Biosciences CenterPostdoctoral fellow - Academic background
2000-04 -- 2005-03 The University of Tokyo School of Engineering Department of Chemistry and Biotechnology 1998-04 -- 2000-03 The University of Tokyo Faculty of Engineering Department of Chemistry and Biotechnology 1996-04 -- 1998-03 The University of Tokyo College of Arts and Sciences: Junior Division Natural Sciences I - Degree
2005-03 博士(工学) The University of Tokyo - Academic societies
2013-01 -- (current) PROTEIN SCIENCE SOCIETY OF JAPAN 2008-04 -- (current) THE SOCIETY OF CEMICAL ENGINEERS, JAPAN 2007-10 -- (current) Japanese Society of Enzyme Engineering 2003-05 -- (current) The Society for Biotechnology, Japan - Honors & Awards
2017-10 酵素工学奨励賞 人為的な酵素の集合化に関する研究 2013-06 研究奨励賞 複数の電子伝達経路を有する人工シトクロムP450複合体 2013-03 研究奨励賞【玉置明善記念賞】 ボトムアップアプローチによる機能性タンパク質複合体の構築 2011-06 FEBS Journal Klaus Ruckpaul poster award Artificial self-sufficient P450: Noncovalent complex with electron transfer components 2011-05 The outstanding award for Young Scientist and Student of oral presentation Noncovalent Complex Formation of a Bacterial Cytochrome P450 and its Electron transfer Proteins by Fusion to PCNA - Articles
- Transition of stability of putidaredoxin reductase by introducing proline
Okamura Taiki; Aritomi Rina; Matsumoto Takuya; Yamada ...
BIOCHEMICAL ENGINEERING JOURNAL/201, 2024-01 - Artificial self-sufficient cytochrome P450 containing multiple auxiliary proteins demonstrates improved monooxygenase activity
Haga Tomoaki; Hirakawa Hidehiko; Nagamune Teruyuki
Biotechnology journal/13(12)/p.1800088, 2018-12 - 水溶性シトクロムP450を固定化酵素として利用するために
平川 秀彦
バイオサイエンスとインダストリー/77(1)/pp.32-36, 2019-01 - リング状ヘテロ三量体タンパク質を利用した人工的な酵素複合体の構築
平川 秀彦; 長棟 輝行
化学と生物/51(8)/pp.521-523, 2013-08 - A stable artificial multienzymatic complex using a heterotrimeric protein from Metallosphaera sedula
Iwata Fumiya; Hirakawa Hidehiko; Nagamune Teruyuki
Biotechnology journal/13(11)/p.1700662, 2018-11 - 逆ミセル中における補酵素再生を伴う酸化還元反応に関する基礎的研究
平川 秀彦; 神谷 典穂; 長棟 輝行
化学工学シンポジウムシリーズ76「高機能界面・分子集合体の基礎構築と応用分野の新展開」/p.133, 2001-06 - Enzyme Logic Gate with Summation Function Based on Putidaredoxin Reductase/Cytochrome c Enzyme Reaction
Yoo Si-Youl; Min Junhong; Haga Tomoaki; Hirakawa Hidehik...
Journal of Nanoscience and Nanotechnology/17(8)/pp.5189-5192, 2017-08 - Immobilization of a Bacterial Cytochrome P450 Monooxygenase System on a Solid Support
Tan Cheau Yuaan; Hirakawa Hidehiko; Suzuki Risa; Haga To...
Angewandte Chemie International Edition/55(48)/pp.15002-15006, 2016-11 - Correlation between Activity and Molecular Structure around the Active Center of Cytochrome P450cam Conjugates
Oshiba Yuhei; Tamaki Takanori; Ohashi Hidenori; Hirakawa ...
Journal of Chemical Engineering of Japan/49(5)/pp.475-480, 2016-05 - Three proliferating cell nuclear antigen homologues from Metallosphaera sedula form a head-to-tail heterotrimer
Iwata Fumiya; Hirakawa Hidehiko; Nagamune Teruyuki
Scientific reports/6(1)/p.6588, 2016-05 - Ca2+-independent sortase-A exhibits high selective protein ligation activity in the cytoplasm of Escherichia coli
Hirakawa Hidehiko; Ishikawa Suguru; Teruyuki Nagamune
Biotechnology Journal/10(9)/pp.1487-1492, 2015-09 - Supramolecular protein assembly supports immobilization of a cytochrome P450 monooxygenase system as water-insoluble gel
Tan Cheau Yuaan; Hirakawa Hidehiko; Teruyuki Nagamune
Scientific Reports/5(1)/p.8648, 2015-03 - Electron donation to an archaeal cytochrome P450 is enhanced by PCNA-mediated selective complex formation with foreign redox proteins
Suzuki Risa; Hirakawa Hidehiko; Teruyuki Nagamune
Biotechnology Journal/9(12)/pp.1573-1581, 2014-12 - Fusion protein bilayer fabrication composed of recombinant azurin/cytochrome P450 by the sortase-mediated ligation method
Lee Taek; Min Junhong; Hirakawa Hidehiko; Nagamune Teruy...
Colloids and Surfaces B: Biointerfaces/120/pp.215-221, 2014-08 - Phosphite-driven Self-sufficient Cytochrome P450
Watanabe Hiroshi; Hirakawa Hidehiko; Nagamune Teruyuki
ChemCatChem/5(12)/pp.3835-3840, 2013-12 - Effect of length of molecular recognition moiety on enzymatic activity switching
Oshiba Yuhei; Tamaki Takanori; Ohashi Hidenori; Hirakawa ...
Journal of Bioscience and Bioengineering/116(4)/pp.433-437, 2013-10 - Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A
Zhang Jing; Yamaguchi Satoshi; Hirakawa Hidehiko; Nagamun...
Journal of Bioscience and Bioengineering/116(3)/pp.298-301, 2013-09 - Fine Tuning of Spatial Arrangement of Enzymes in a PCNA-Mediated Multienzyme Complex Using a Rigid Poly-L-Proline Linker
Haga Tomoaki; Hirakawa Hidehiko; Nagamune Teruyuki
PLoS ONE/8(9)/p.e75114, 2013-09 - Introduction of selective intersubunit disulfide bonds into self-assembly protein scaffold to enhance an artificial multienzyme complex's activity
Hirakawa Hidehiko; Kakitani Ayano; Nagamune Teruyuki
Biotechnology and Bioengineering/110(7)/pp.1858-1864, 2013-07 - Molecular recognition moiety and its target biomolecule interact in switching enzyme activity
Oshiba Yuhei; Tamaki Takanori; Ohashi Hidenori; Hirakawa ...
Journal of Bioscience and Bioengineering/115(6)/pp.639-644, 2013-06 - Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
Hirakawa Hidehiko; Ishikawa Suguru; Nagamune Teruyuki
Biotechnology and Bioengineering/109(12)/pp.2955-2961, 2012-12 - Artificial Protein Complexes for Biocatalysis
Hirakawa Hidehiko; Haga Tomoaki; Nagamune Teruyuki
Topics in Catalysis/55(16-18)/pp.1124-1137, 2012-11 - Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site
Yamamura Yuichi; Hirakawa Hidehiko; Yamaguchi Satoshi; Na...
Chemical Communications/47(16)/pp.4742-4744, 2011-04 - Crystal structure of histamine dehydrogenase from Nocardioides simplex
Reed Timothy; Lushington Gerald H.; Xia Yan; Hirakawa Hi...
Journal of Biological Chemistry/285(33)/pp.25782-25791, 2010-08 - Molecular Assembly of P450 with Ferredoxin and Ferredoxin Reductase by Fusion to PCNA
Hirakawa Hidehiko; Nagamune Teruyuki
ChemBioChem/11(11)/pp.1517-1520, 2010-07 - more...
- Transition of stability of putidaredoxin reductase by introducing proline
- Books
- ヘテロ三量体タンパク質を利用したシトクロムP450モノオキシゲナーゼの効果的固定化
平川 秀彦
Immobilization of Cells and Biomolecules for Functional Fabrication/シーエムシー出版/pp.10-17, 2018-04 - Use of sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes
Hirakawa Hidehiko; Nagamune Teruyuki
Enzyme Engineering/pp.149-163, 2013-01 - Nanoscale-engineered cytochrome p450 system with a branch structure
Hirakawa Hidehiko; Nagamune Teruyuki
Nanoscale Biocatalysis/pp.1-16, 2011-01 - Development of enzymatic catalysts working in nonaqueous media
Hirakawa Hidehiko
2005-03
- ヘテロ三量体タンパク質を利用したシトクロムP450モノオキシゲナーゼの効果的固定化
- Conference, etc.
- 酵素本来の力を引き出すためのアプローチ
平川 秀彦
第24回食品微細科学研究会講演会/2023-09-07 - マルチウェルプレートを用いた Pseudomonas putida 由来シトクロム P450 と電子伝達タンパク質間相互作用の評価
林 幹太; 平川 秀彦
酵素工学研究会第90回講演会/2023-11-10 - Metallosphaera sedula 由来 PCNA を利用したペプチド転移反応の制御
多田 修平; 平川 秀彦
酵素工学研究会第90回講演会/2023-11-10 - Detection of interactions between cytochrome P450 and redox proteins using a Metallosphaera sedula scaffold protein
Kuribayashi Kanta; Hirakawa Hidehiko
2023 International Joint Meeting of the 23rd International Conference on Cytochrome P450 and the 38th Annual Meeting of the Japanese Society for the Study of Xenobiotics (2023 ICCP450/JSSX)/2023-09-27 - Biotechnological applications based on insights from artificial assembly of cytochrome P450 and redox proteins
Hirakawa Hidehiko
2023 International Joint Meeting of the 23rd International Conference on Cytochrome P450 and the 38th Annual Meeting of the Japanese Society for the Study of Xenobiotics (2023 ICCP450/JSSX)/2023-09-27 - Artificial proximity of electron transfer protein to cytochrome P450 opens a treasure chest"
Hirakawa Hidehiko
The 2021 International Chemical Congress of Pacific Basin Societies (Pacifichem 2021)/2021-12-16 - Unorthodox Approach to Generate Self-sufficient Cytochrome P450
Hirakawa Hidehiko
10th Asian Biological Biological Inorganic Chemistry Conference/2022-12-02 - Metallosphaera sedula由来核内増殖抗原を利用したシトクロムP450システムの不溶化
渡邊 千晶; 平川 秀彦; 市川 創作
酵素工学研究会第82回講演会/2019-11-01--2019-11-01 - 人為的な近接化を利用したシトクロムP450camープチダレドキシン間相互作用の検出
齋藤 航汰; 平川 秀彦; 市川 創作
酵素工学研究会第82回講演会/2019-11-01--2019-11-01 - Protein engineering for practical enzymatic protein/peptide ligation
Hirakawa Hidehiko
第19回日本蛋白質科学会年会 第71回日本細胞生物学会大会 合同年次大会/2019-06-24--2019-06-24 - 複数の酸化還元ドメインを有する人工自己充足型シトクロムP450における電子伝達プロセスの解析
平川 秀彦
第31回生物無機化学夏季セミナー/2018-09-13--2018-09-13 - What is happening in an artificial self-sufficient cytochrome P450?
Hirakawa Hidehiko
The 15th Japan-China-Korea Joint Symposium on Enzyme Engineering/2018-06-30--2018-07-02 - 人為的な酵素の集合化に関する研究
Hirakawa Hidehiko
酵素工学研究会第78回講演会/2017-10-06--2017-10-06 - 人工自己充足型シトクロムP450の速度論的解析
芳賀 智亮; 平川 秀彦; 長棟 輝行
化学工学会第49回秋季大会/2017-09-21--2017-09-21 - 逐次的な多酵素複合体構築
岩田 史也; 平川 秀彦; 長棟 輝行
第69回日本生物工学会大会/2017-09-12--2017-09-12 - Benefits from artificial assembly with redox partners
Hirakawa Hidehiko
20th International Conference on Cytochrome P450: Biochemistry, Biophysics and Biotechnology/2017-08-27--2017-08-31 - 配列特異的なDNA結合タンパク質を用いた細菌由来シトクロムP450システムの再構成
畠山 洋平; 平川 秀彦; 長棟 輝行
化学工学会第82年会/2017-03-07--2017-03-07 - Competitive detection of interaction between cytochrome P450 and electron transfer protein
Kondo Masayoshi; Hirakawa Hidehiko; Nagamune Teruyuki
The Fifth International Conference on Cofactors and Active Enzyme Molecule 2016/2016-09-07--2016-09-07 - 黄色ブドウ球菌由来ペプチド転移酵素における基質阻害機構の解明と新規変異体の開発
畠山 美沙樹; 平川 秀彦; 長棟 輝行
化学工学会群馬大会2015/2015-11-27--2015-11-27 - Artificial Assembly of Cytochrome P450 Monooxygenase and Auxiliary Proteins for Efficient Electron Supply
Hirakawa Hidehiko
錯体化学会第65回討論会/2015-09-21--2015-09-23 - An artificial self-sufficient cytochrome P450 wakes up earlier with extra redox components
Haga Tomoaki; Hirakawa Hidehiko; Nagamune Teruyuki
Active Enzyme Molecule 2014/2014-12-19--2014-12-19 - Identification of the electron transfer partner proteins that can activate P450 from Thermobifida fusca
Kondo Masayoshi; Hirakawa Hidehiko; Nagamune Teruyuki
Active Enzyme Molecule 2014/2014-12-19--2014-12-19 - Design of an artificial cellulolytic multi-enzyme complex aligned by PTDH-linked PCNA heterotrimer units
Schmitt Katharina; Hirakawa Hidehiko; Nagamune Teruyuki
Active Enzyme Molecule 2014/2014-12-17--2014-12-17 - Immobilization of cytochrome P450 and its redox protein partners coupled with cofactor regeneration
Tan Cheau Yuaan; 渡辺 浩志; 平川 秀彦; 長棟 輝行
化学工学会第79年会/2014-03-30--2014-03-30 - PCNA heterotrimer mediated cluster formation of an artificial cellulosome complex
Schmitt Katharina; 平川 秀彦; 長棟 輝行
化学工学会第79年会/2014-03-18--2014-03-18 - more...
- 酵素本来の力を引き出すためのアプローチ
- Teaching
2024-04 -- 2024-08 Seminar in Applied Biochemistry IIF(Spring) University of Tsukuba. 2024-04 -- 2024-08 Research in Applied Biochemistry IIF(Spring) University of Tsukuba. 2024-10 -- 2025-03 Doctoral Research in Life Science Innovation Ⅰ University of Tsukuba. 2024-10 -- 2025-03 Master's Research in Life Science Innovation Ⅲ University of Tsukuba. 2024-10 -- 2025-03 Master's Research in Life Science Innovation Ⅳ University of Tsukuba. 2024-04 -- 2024-08 Seminar in Life and Agricultural Sciences University of Tsukuba. 2024-10 -- 2025-02 Seminar in Life and Agricultural Sciences University of Tsukuba. 2024-10 -- 2025-03 Doctoral Research in Life Science Innovation Ⅴ University of Tsukuba. 2024-10 -- 2025-03 Life Science Innovation Master's Special Seminar I Fall University of Tsukuba. 2024-10 -- 2025-03 Doctoral Research in Life Science Innovation Ⅱ University of Tsukuba. more... - Talks
- 酵素本来の力を引き出すためのアプローチ
平川 秀彦
第24回食品微細科学研究会講演会/2023-09-07 - 酵素のかたちとはたらき
平川 秀彦
くらしの中の生化学-生きもののしくみを知るー/2022-08-27 - Immobilization of Soluble Cytochrome P450 Monooxygenase
Hirakawa Hidehiko
T-LSI Symposium on Life Science Innovation and Biomaterials/2018-09-05--2018-09-05
- 酵素本来の力を引き出すためのアプローチ
- Professional activities
2023-04 -- 2025-03 The Society for Bioscience and Bioengineering, Japan 代議員 2021-06 -- (current) Journal of Bioscience and Bioengineering Editorial Board Member 2020-05 -- (current) Applied Sciences Editorial Board Member 2019-01 -- (current) Japanese Society of Enzyme Engineering 幹事 2015-05 -- (current) Scientific Reports (Nature Publishing Group) Editorial Board Member - University Management
2021-05 -- (current) 遺伝子組換え実験安全委員会 委員 2018-04 -- (current) ライフイノベーション学位プログラム運営委員会 委員 2019-04 -- 2019-11 ライフイノベーション学位プログラム広報・連携委員会 委員長 2018-04 -- (current) ライフイノベーション学位プログラムFD委員会 委員長 2018-04 -- (current) ライフイノベーション学位プログラム教務委員会 委員長 2018-04 -- 2019-03 ライフイノベーション学位プログラム広報・連携委員会 委員 - Other activities
2018-09 -- 2018-09 T-LSI Symposium on Life Science Innovation and Biomaterialsを開催
(Last updated: 2024-08-20)